Application
Neutralization (see biological activity)
Biochem/physiol Actions
EC50 = 100-300 nM based on its ability to inhibit 54 nM caspase-8-cleaved human Bid-mediated release of cytochrome c from isolated mouse liver mitochondria
General description
Recombinant, human Bcl-2 without the 17 amino acid mitochondrial targeting sequence at the C-terminus, fused to a His Tag™ sequence, and expressed in E. coli. The truncated protein retains the ability to neutralize pro-apoptotic Bcl-2 family members.
Recombinant, human Bcl-2 without the 17 amino acid mitochondrial targeting sequence at the C-terminus, fused to a His-Tag™ sequence and expressed in E. coli. The truncated protein retains the ability to neutralize pro-apoptotic Bcl-2 family members.
Legal Information
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Packaging
50 µg in Glass bottle
Please refer to vial label for lot-specific concentration.
Physical form
In 100 mM KCl, 10 mM HEPES, 20% glycerol, pH 8.0.
Reconstitution
Following initial thaw, aliquot and freeze (-20°C).
Warning
Toxicity: Standard Handling (A)
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